immpact novared detection reagent Search Results


immpact dab substrate  (Vector Laboratories)
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Vector Laboratories immpact dab substrate
Immpact Dab Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immpact novared  (Vector Laboratories)
96
Vector Laboratories immpact novared
Immpact Novared, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immpact novared - by Bioz Stars, 2026-07
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vector immpact novared peroxidase substrate kit  (Vector Laboratories)
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Vector Laboratories vector immpact novared peroxidase substrate kit
Vector Immpact Novared Peroxidase Substrate Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vector immpact novared peroxidase substrate kit - by Bioz Stars, 2026-07
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vectastain abc elite kit  (Vector Laboratories)
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Vector Laboratories vectastain abc elite kit
Vectastain Abc Elite Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vectastain abc elite kit - by Bioz Stars, 2026-07
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immpact novared® substrate  (IMMPACT Biotechnologies GmbH)
90
IMMPACT Biotechnologies GmbH immpact novared® substrate
a , b SH-SY5Y cells were transiently co-transfected with Myc-DJ1 and FLAG-CRBN. Cells were lysed and incubated with scramble or DJ1-based tetrapeptide inhibitor DGRT with both ends blocked (Ac-DGRT-NH 2 ). CoIP was performed with α-FLAG antibody. c , d WT mice were injected with PFF as described before alongwith the stereotaxic ICV injection of DGRT or scrambled peptide (10.3 μL of 10 mM stock to inject 50 μg). e , <t>f</t> <t>IHC</t> staining of substantia nigra of brain from WT mice using ImmPACT <t>NovaRED</t> ® substrate (upper panel) and alexa-conjugated antibody for pSer129 α-SYN staining. Data was analyzed by student’s t test and presented as mean ± SEM. Experiments were performed 6 times. * p < 0.05, ** p < 0.01, and *** p < 0.001. g Aggregation assays for α-SYN monomers incubated with mentioned combinations with 2 μM Hsp70, 1 μM APG2 and 2 mM ATP added to all the samples, incubated at 30 °C for 150 h with gentle rotatory shaking. Ac-DGRT-NH 2 inhibited the effects of CRBN as shown by green line.
Immpact Novared® Substrate, supplied by IMMPACT Biotechnologies GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immpact novared® substrate - by Bioz Stars, 2026-07
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aec hrp substrate  (Vector Laboratories)
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Vector Laboratories aec hrp substrate
a , b SH-SY5Y cells were transiently co-transfected with Myc-DJ1 and FLAG-CRBN. Cells were lysed and incubated with scramble or DJ1-based tetrapeptide inhibitor DGRT with both ends blocked (Ac-DGRT-NH 2 ). CoIP was performed with α-FLAG antibody. c , d WT mice were injected with PFF as described before alongwith the stereotaxic ICV injection of DGRT or scrambled peptide (10.3 μL of 10 mM stock to inject 50 μg). e , <t>f</t> <t>IHC</t> staining of substantia nigra of brain from WT mice using ImmPACT <t>NovaRED</t> ® substrate (upper panel) and alexa-conjugated antibody for pSer129 α-SYN staining. Data was analyzed by student’s t test and presented as mean ± SEM. Experiments were performed 6 times. * p < 0.05, ** p < 0.01, and *** p < 0.001. g Aggregation assays for α-SYN monomers incubated with mentioned combinations with 2 μM Hsp70, 1 μM APG2 and 2 mM ATP added to all the samples, incubated at 30 °C for 150 h with gentle rotatory shaking. Ac-DGRT-NH 2 inhibited the effects of CRBN as shown by green line.
Aec Hrp Substrate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aec hrp substrate - by Bioz Stars, 2026-07
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chromogenic substrate  (IMMPACT Biotechnologies GmbH)
90
IMMPACT Biotechnologies GmbH chromogenic substrate
a , b SH-SY5Y cells were transiently co-transfected with Myc-DJ1 and FLAG-CRBN. Cells were lysed and incubated with scramble or DJ1-based tetrapeptide inhibitor DGRT with both ends blocked (Ac-DGRT-NH 2 ). CoIP was performed with α-FLAG antibody. c , d WT mice were injected with PFF as described before alongwith the stereotaxic ICV injection of DGRT or scrambled peptide (10.3 μL of 10 mM stock to inject 50 μg). e , <t>f</t> <t>IHC</t> staining of substantia nigra of brain from WT mice using ImmPACT <t>NovaRED</t> ® substrate (upper panel) and alexa-conjugated antibody for pSer129 α-SYN staining. Data was analyzed by student’s t test and presented as mean ± SEM. Experiments were performed 6 times. * p < 0.05, ** p < 0.01, and *** p < 0.001. g Aggregation assays for α-SYN monomers incubated with mentioned combinations with 2 μM Hsp70, 1 μM APG2 and 2 mM ATP added to all the samples, incubated at 30 °C for 150 h with gentle rotatory shaking. Ac-DGRT-NH 2 inhibited the effects of CRBN as shown by green line.
Chromogenic Substrate, supplied by IMMPACT Biotechnologies GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chromogenic substrate - by Bioz Stars, 2026-07
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peroxidase substrate method dabimmpact  (Vector Laboratories)
95
Vector Laboratories peroxidase substrate method dabimmpact
a , b SH-SY5Y cells were transiently co-transfected with Myc-DJ1 and FLAG-CRBN. Cells were lysed and incubated with scramble or DJ1-based tetrapeptide inhibitor DGRT with both ends blocked (Ac-DGRT-NH 2 ). CoIP was performed with α-FLAG antibody. c , d WT mice were injected with PFF as described before alongwith the stereotaxic ICV injection of DGRT or scrambled peptide (10.3 μL of 10 mM stock to inject 50 μg). e , <t>f</t> <t>IHC</t> staining of substantia nigra of brain from WT mice using ImmPACT <t>NovaRED</t> ® substrate (upper panel) and alexa-conjugated antibody for pSer129 α-SYN staining. Data was analyzed by student’s t test and presented as mean ± SEM. Experiments were performed 6 times. * p < 0.05, ** p < 0.01, and *** p < 0.001. g Aggregation assays for α-SYN monomers incubated with mentioned combinations with 2 μM Hsp70, 1 μM APG2 and 2 mM ATP added to all the samples, incubated at 30 °C for 150 h with gentle rotatory shaking. Ac-DGRT-NH 2 inhibited the effects of CRBN as shown by green line.
Peroxidase Substrate Method Dabimmpact, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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peroxidase substrate method dabimmpact - by Bioz Stars, 2026-07
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immpact novared kit  (Vector Laboratories)
96
Vector Laboratories immpact novared kit
a , b SH-SY5Y cells were transiently co-transfected with Myc-DJ1 and FLAG-CRBN. Cells were lysed and incubated with scramble or DJ1-based tetrapeptide inhibitor DGRT with both ends blocked (Ac-DGRT-NH 2 ). CoIP was performed with α-FLAG antibody. c , d WT mice were injected with PFF as described before alongwith the stereotaxic ICV injection of DGRT or scrambled peptide (10.3 μL of 10 mM stock to inject 50 μg). e , <t>f</t> <t>IHC</t> staining of substantia nigra of brain from WT mice using ImmPACT <t>NovaRED</t> ® substrate (upper panel) and alexa-conjugated antibody for pSer129 α-SYN staining. Data was analyzed by student’s t test and presented as mean ± SEM. Experiments were performed 6 times. * p < 0.05, ** p < 0.01, and *** p < 0.001. g Aggregation assays for α-SYN monomers incubated with mentioned combinations with 2 μM Hsp70, 1 μM APG2 and 2 mM ATP added to all the samples, incubated at 30 °C for 150 h with gentle rotatory shaking. Ac-DGRT-NH 2 inhibited the effects of CRBN as shown by green line.
Immpact Novared Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/immpact+novared+detection+reagent/pm32443499-69-22-25?v=Vector+Laboratories
Average 96 stars, based on 1 article reviews
immpact novared kit - by Bioz Stars, 2026-07
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amec red substrate  (IMMPACT Biotechnologies GmbH)
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IMMPACT Biotechnologies GmbH amec red substrate
a , b SH-SY5Y cells were transiently co-transfected with Myc-DJ1 and FLAG-CRBN. Cells were lysed and incubated with scramble or DJ1-based tetrapeptide inhibitor DGRT with both ends blocked (Ac-DGRT-NH 2 ). CoIP was performed with α-FLAG antibody. c , d WT mice were injected with PFF as described before alongwith the stereotaxic ICV injection of DGRT or scrambled peptide (10.3 μL of 10 mM stock to inject 50 μg). e , <t>f</t> <t>IHC</t> staining of substantia nigra of brain from WT mice using ImmPACT <t>NovaRED</t> ® substrate (upper panel) and alexa-conjugated antibody for pSer129 α-SYN staining. Data was analyzed by student’s t test and presented as mean ± SEM. Experiments were performed 6 times. * p < 0.05, ** p < 0.01, and *** p < 0.001. g Aggregation assays for α-SYN monomers incubated with mentioned combinations with 2 μM Hsp70, 1 μM APG2 and 2 mM ATP added to all the samples, incubated at 30 °C for 150 h with gentle rotatory shaking. Ac-DGRT-NH 2 inhibited the effects of CRBN as shown by green line.
Amec Red Substrate, supplied by IMMPACT Biotechnologies GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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peroxidase substrate  (IMMPACT Biotechnologies GmbH)
90
IMMPACT Biotechnologies GmbH peroxidase substrate
a , b SH-SY5Y cells were transiently co-transfected with Myc-DJ1 and FLAG-CRBN. Cells were lysed and incubated with scramble or DJ1-based tetrapeptide inhibitor DGRT with both ends blocked (Ac-DGRT-NH 2 ). CoIP was performed with α-FLAG antibody. c , d WT mice were injected with PFF as described before alongwith the stereotaxic ICV injection of DGRT or scrambled peptide (10.3 μL of 10 mM stock to inject 50 μg). e , <t>f</t> <t>IHC</t> staining of substantia nigra of brain from WT mice using ImmPACT <t>NovaRED</t> ® substrate (upper panel) and alexa-conjugated antibody for pSer129 α-SYN staining. Data was analyzed by student’s t test and presented as mean ± SEM. Experiments were performed 6 times. * p < 0.05, ** p < 0.01, and *** p < 0.001. g Aggregation assays for α-SYN monomers incubated with mentioned combinations with 2 μM Hsp70, 1 μM APG2 and 2 mM ATP added to all the samples, incubated at 30 °C for 150 h with gentle rotatory shaking. Ac-DGRT-NH 2 inhibited the effects of CRBN as shown by green line.
Peroxidase Substrate, supplied by IMMPACT Biotechnologies GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immpact (tm) novared (tm) hrp substrate  (Novus Biologicals)
N/A
ImmPACT (TM) NovaRED (TM) HRP Substrate
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a , b SH-SY5Y cells were transiently co-transfected with Myc-DJ1 and FLAG-CRBN. Cells were lysed and incubated with scramble or DJ1-based tetrapeptide inhibitor DGRT with both ends blocked (Ac-DGRT-NH 2 ). CoIP was performed with α-FLAG antibody. c , d WT mice were injected with PFF as described before alongwith the stereotaxic ICV injection of DGRT or scrambled peptide (10.3 μL of 10 mM stock to inject 50 μg). e , f IHC staining of substantia nigra of brain from WT mice using ImmPACT NovaRED ® substrate (upper panel) and alexa-conjugated antibody for pSer129 α-SYN staining. Data was analyzed by student’s t test and presented as mean ± SEM. Experiments were performed 6 times. * p < 0.05, ** p < 0.01, and *** p < 0.001. g Aggregation assays for α-SYN monomers incubated with mentioned combinations with 2 μM Hsp70, 1 μM APG2 and 2 mM ATP added to all the samples, incubated at 30 °C for 150 h with gentle rotatory shaking. Ac-DGRT-NH 2 inhibited the effects of CRBN as shown by green line.

Journal: NPJ Parkinson's Disease

Article Title: CRBN modulates synuclein fibrillation via degradation of DNAJB1 in mouse model of Parkinson disease

doi: 10.1038/s41531-024-00801-3

Figure Lengend Snippet: a , b SH-SY5Y cells were transiently co-transfected with Myc-DJ1 and FLAG-CRBN. Cells were lysed and incubated with scramble or DJ1-based tetrapeptide inhibitor DGRT with both ends blocked (Ac-DGRT-NH 2 ). CoIP was performed with α-FLAG antibody. c , d WT mice were injected with PFF as described before alongwith the stereotaxic ICV injection of DGRT or scrambled peptide (10.3 μL of 10 mM stock to inject 50 μg). e , f IHC staining of substantia nigra of brain from WT mice using ImmPACT NovaRED ® substrate (upper panel) and alexa-conjugated antibody for pSer129 α-SYN staining. Data was analyzed by student’s t test and presented as mean ± SEM. Experiments were performed 6 times. * p < 0.05, ** p < 0.01, and *** p < 0.001. g Aggregation assays for α-SYN monomers incubated with mentioned combinations with 2 μM Hsp70, 1 μM APG2 and 2 mM ATP added to all the samples, incubated at 30 °C for 150 h with gentle rotatory shaking. Ac-DGRT-NH 2 inhibited the effects of CRBN as shown by green line.

Article Snippet: CoIP was performed with α-FLAG antibody. c , d WT mice were injected with PFF as described before alongwith the stereotaxic ICV injection of DGRT or scrambled peptide (10.3 μL of 10 mM stock to inject 50 μg). e , f IHC staining of substantia nigra of brain from WT mice using ImmPACT NovaRED ® substrate (upper panel) and alexa-conjugated antibody for pSer129 α-SYN staining.

Techniques: Transfection, Incubation, Injection, Immunohistochemistry, Staining, Gentle

Reagents and peptides used in the study

Journal: NPJ Parkinson's Disease

Article Title: CRBN modulates synuclein fibrillation via degradation of DNAJB1 in mouse model of Parkinson disease

doi: 10.1038/s41531-024-00801-3

Figure Lengend Snippet: Reagents and peptides used in the study

Article Snippet: CoIP was performed with α-FLAG antibody. c , d WT mice were injected with PFF as described before alongwith the stereotaxic ICV injection of DGRT or scrambled peptide (10.3 μL of 10 mM stock to inject 50 μg). e , f IHC staining of substantia nigra of brain from WT mice using ImmPACT NovaRED ® substrate (upper panel) and alexa-conjugated antibody for pSer129 α-SYN staining.

Techniques: Protease Inhibitor, Plasmid Preparation, Recombinant